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rabbit polyclonals against zo2  (Thermo Fisher)


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    Thermo Fisher rabbit polyclonals against zo2
    Rabbit Polyclonals Against Zo2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonals+against+zo2/pm28554775-55-15-19
    Average 86 stars, based on 1 article reviews
    rabbit polyclonals against zo2 - by Bioz Stars, 2026-10
    86/100 stars

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    Immunofluorescence:

    Article Title: Zonula occludens-2 regulates Rho proteins activity and the development of epithelial cytoarchitecture and barrier function.
    Article Snippet: ZO-2 parental cells plated at sparse density were transfected with LipofectamineTM 2000 with 0.5 μg of p-EGFP (Clontech, Cat. 6085-1, Palo Alto, CA) and 0.4 μg of construct pCDNA-EGFP-GEF-H1 C53R (generously provided by Dr. Celine DerMardirossian). .. Immunofluorescence was done following standard procedures as described previously [38] using the following primary antibodies: rabbit polyclonals against ZO2 (Invitrogen, Cat. 711400, dilution 1:100, Carlsbad, CA), myosin IIa (Covance, Cat. PRB-445P; dilution 1:100, Berkeley, CA), phospho myosin light chain kinase 2 (pMLC2 Ser 20) (Abcam, Cat. Ab2480, dilution 1:100, Cambridge, MA), β-tubulin coupled to FITC (Sigma Aldrich, Cat. F2043, dilution 1:50, St. Louis, MO), claudin-1 (Invitrogen, Cat. 51-9000, dilution 1:50, Camarillo, CA) and claudin-7 (Invitrogen, Cat. 34- 9100 ̧dilution 1:50, Camarillo, CA); and mouse monoclonals against gp135/podocalyxin (Invitrogen, Cat. 39-3800, dilution 1:100, Camarillo, CA), GEF-H1 (Abcam, Cat. Ab90783, dilution 1:5, Cambridge, MA), and β-tubulin (Sigma Aldrich, Cat. T7816; dilution 1:5000, St Louis, MO), as well as a rat monoclonal against Ecadherin (Sigma-Aldrich, Cat U3254; dilution 1:100, St Louis, MO), a rabbit monoclonal against p-cofilin (Ser3) (Cell Signaling Technology, Cat. 3313; dilution 1:1000; Danvers, MA) and a mouse monoclonal against β-tubulin (sigma-Aldrich, Cat T7816; dilution 1:500, St Louis, MO). .. As secondary antibodies we employed donkey AC C EP TE D M AN U SC R IP T antibodies against rabbit IgG coupled to Alexa Fluor 594 (Invitrogen, Cat A21207, dilution 1:100, Carlsbad, CA) and against mouse IgG coupled to Alexa Fluor 594 (Invitrogen, Cat A21203, dilution 1:100, Carlsbad, CA) or to Alexa Fluor 488 (Life Technologies, Cat A11001, dilution 1:100, Eugene, OR), as well as a rabbit antibody against rat IgG coupled to FITC (Sigma-Aldrich, Cat. F1763, St. Louis MO).



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    rabbit polyclonals against zo2  (Thermo Fisher)
    86
    Thermo Fisher rabbit polyclonals against zo2
    Rabbit Polyclonals Against Zo2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonals+against+zo2/pm28554775-55-15-19
    Average 86 stars, based on 1 article reviews
    rabbit polyclonals against zo2 - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier

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